Journal: iScience

Article Title: A genome-wide CRISPR screen maps endogenous regulators of PPARG gene expression in bladder cancer

doi: 10.1016/j.isci.2023.106525

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Article Snippet: pMD2.G - VSV-G envelope expressing plasmid , Addgene , Cat# 12259.

Techniques: Produced, Virus, Recombinant, Protease Inhibitor, Transfection, Cell Viability Assay, Purification, CRISPR, Sequencing, Biomarker Discovery, Plasmid Preparation, Expressing, Knock-Out, Control, Software

Journal: Cell

Article Title: Elimination of Toxic Microsatellite Repeat Expansion RNA by RNA-Targeting Cas9

doi: 10.1016/j.cell.2017.07.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti- Mbnl1 Gift from Maurice Swanson A2764 Critical Commercial Assays NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78833 MAXIscript SP6 in vitro transcription kit Thermo Fisher Scientific Cat#AM1308 TruSeq Stranded mRNA Library Prep Kit Illumina Cat#RS-122-2101 Deposited Data Data reported in this paper N/A GEO: {"type":"entrez-geo","attrs":{"text":"GSE100943","term_id":"100943"}} GSE100943 Experimental Models: Cell Lines DM1 patient myoblasts Gift from Guangbin Xia N/A Oligonucleotides FISH probes See Table S1 N/A PCR primers See Table S5 N/A Recombinant DNA Plasmid: psPAX2 Gift from Didier Trono Addgene Plasmid #12260 Plasmid: pCMV-VSV-G Gift from Bob Weinberg ( Stewart et al., 2003 ) Addgene Plasmid #8454 Plasmid: DT960 Gift from Thomas Cooper ( Lee et al., 2012 ) TRE-CTG960 Plasmid: LentiRCas9 (EFS-PIN-dCas9 and U6-sgRNA) This paper N/A (to be deposited in Addgene) Plasmid: pCDNA 3.1 PIN-dCas9 This paper N/A (to be deposited in Addgene) Plasmid: pBluescript SKII U6-sgRNA See Table S2 for sgRNA spacers and scaffold sequences N/A (to be deposited in Addgene) Software and Algorithms OLego Zhang lab ( https://zhanglab.c2b2.columbia.edu/index.php/OLego ) N/A Quantas Zhang lab ( https://zhanglab.c2b2.columbia.edu/index.php/Quantas_Documentation#Download ) N/A Open in a separate window KEY RESOURCES TABLE

Techniques: In Vitro, Recombinant, Plasmid Preparation, Software